Dual color live-cell imaging of opto-kinesin and iLID mCherry-RAB5 . Related to Fig. 4. (A) Live-cell imaging of iLID-mCherry-RAB5 and opto-kinesin KIF1A(1–365)-VVDfast-mVenus-SSPB(micro) in U2OS before and after 10 min of illumination (see also Video 4). Scale bar is 10 µm. (B) Kymograph showing early endosome motility and induced colocalization with opto-kinesin as in A. (C) Example of induced early endosome motility (marked by arrowheads) as in A, before and immediately after illumination. Scale bar is 5 µm.