Increased MT stability/length rescues bipolar spindle formation in polyploid OVCAR-8 cells. (A) Representative images of Cytosim-based simulations of spindle formation. (B) Graph bars of the percentage of simulations in each category (n = 30 simulations/condition). (C and D) Stills of time-lapse videos of mitotic polyploid OVCAR-8 cells with stabilized MTs (siMCAK) expressing histone 2B-GFP (red and gray in the middle insets) and incubated with SiR-tubulin (green and fire LUT in the bottom insets). Orange and white dotted circles surround cells and nuclei, respectively. Time of mitosis is represented in minutes:seconds. Time 00:00 corresponds to NEBD. (E) Graph bars showing the percentage of cells in each category in Ctrl, siCtrl (n = 96 cells from two independent experiments), Ctrl, siMCAK (n = 100 cells from two independent experiments), polyploid, siCtrl (n = 81 cells from two independent experiments), and polyploid, siMCAK (n = 109 cells from two independent experiments) OVCAR-8 cells. Statistical significance by a multiple t test. p represents the P value between “multipolar” populations. (F) Graph bars showing the percentage of (pseudo-)bipolar siMCAK, polyploid OVCAR-eight cells presenting an improvement of centrosome clustering or spindle pole coalescence. Error bars represent the mean ± SD.