Polyploid OVCAR-8 cells undergo multipolar divisions regardless the number of nuclei at mitotic entry. (A) Representative images of Cytosim-based simulations of spindle formation. (B) Graph bars of the percentage of simulations in each category (n = 30 simulations/condition). (C) Stills of time-lapse video of mitotic mononucleated polyploid OVCAR-8 cell expressing histone 2B-GFP (red and gray in the middle insets) and incubated with SiR-tubulin (green and fire LUT in the bottom insets). Orange and white dotted circles surround cells and nuclei, respectively. Time of mitosis is represented in minutes:seconds. Time 00:00 corresponds to NEBD. (D) Graph bars of the percentage of cells in each category in Ctrl (n = 152 cells from three independent experiments) and mononucleated polyploid OVCAR-8 cells (n = 178 cells from three independent experiments). Statistical significance by a multiple t test. Error bars represent the mean ± SD and p the P value between “multipolar” populations.