Figure 7.
Chromosome laser ablation allows spindle pole coalescence in polyploid OVCAR-8 cells.(A–D) Stills of time-lapse videos of mitotic binucleated polyploid OVCAR-8 cells expressing histone 2B-GFP (red and gray in the middle insets) and incubated with SiR-tubulin (green and fire LUT in the bottom insets). Yellow dotted circles surround the laser ablated area. Time of mitosis is represented in minutes:seconds. Time 00:00 corresponds to the time of laser ablation. (E) Graph bars representing the percentage of laser ablation events leading to the indicated behaviors (n = 74 cells from six independent experiments). (F) Dot plot representing the distance between the two poles on both sides of laser ablated DNA in polyploid OVCAR-8 cells (n = 74 cells). Statistical significance by a Mann-Whitney test. Error bars represent the mean ± SD and p the P value.

Chromosome laser ablation allows spindle pole coalescence in polyploid OVCAR-8 cells. (A – D) Stills of time-lapse videos of mitotic binucleated polyploid OVCAR-8 cells expressing histone 2B-GFP (red and gray in the middle insets) and incubated with SiR-tubulin (green and fire LUT in the bottom insets). Yellow dotted circles surround the laser ablated area. Time of mitosis is represented in minutes:seconds. Time 00:00 corresponds to the time of laser ablation. (E) Graph bars representing the percentage of laser ablation events leading to the indicated behaviors (n = 74 cells from six independent experiments). (F) Dot plot representing the distance between the two poles on both sides of laser ablated DNA in polyploid OVCAR-8 cells (n = 74 cells). Statistical significance by a Mann-Whitney test. Error bars represent the mean ± SD and p the P value.

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