Polyploid OVCAR-8 cells also divide multipolarly and fail spindle pole coalescence. (A and B) Images of Ctrl (A) and polyploid (B) OVCAR-8 cells labeled with antibodies against α-tubulin (red and gray) and CEP192 (green) to label the mitotic spindle and the PCM, respectively. DNA in blue and gray. (C and D) Stills of time-lapse videos of mitotic binucleated polyploid OVCAR-8 cells expressing Histone 2B-GFP (red and gray in the middle insets) and incubated with SiR-tubulin (green and fire LUT in the bottom insets). Orange and white dotted circles surround cells and nuclei, respectively. Time of mitosis is represented in minutes:seconds. Time 00:00 corresponds to NEBD. (E) Graph bars of the percentage of cells in each category in Ctrl (n = 142 cells from three independent experiments) and binucleated polyploid OVCAR-8 cells (n = 101 cells from three independent experiments). Statistical significance by a multiple t test. Error bars represent the mean ± SD and p the P value between “multipolar” populations. (F) Dot plot showing the area of revolved (white) and unresolved (yellow) chromosome barriers in 21 polyploid OVCAR-8 cells. Stills of cell 14 (bold) shown in D.