Figure 5.
OVCAR-8 cells are proficient for centrosome clustering.(A and B) Stills of time-lapse videos of mitotic Ctrl (DOX-; A) and centrosome amplified PLK4OE (DOX+; B) OVCAR-8 cells expressing Histone 2B-RFP (red and gray in the middle insets) and incubated with SiR-tubulin (green and fire lookup table; LUT in the bottom insets). Orange and white dotted circles surround cells and nuclei, respectively. Time of mitosis is represented in minutes:seconds. Time 00:00 corresponds to the first NEBD. (C) Graph bars showing the percentage of cells in Ctrl (n = 149 cells from two independent experiments) and PLK4OE (n = 161 cells from two independent experiments) OVCAR-8 cells. Statistical significance by a multiple t test. Error bars represent the mean ± SD, p the P value; ns, not significant.

OVCAR-8 cells are proficient for centrosome clustering. (A and B) Stills of time-lapse videos of mitotic Ctrl (DOX-; A) and centrosome amplified PLK4OE (DOX+; B) OVCAR-8 cells expressing Histone 2B-RFP (red and gray in the middle insets) and incubated with SiR-tubulin (green and fire lookup table; LUT in the bottom insets). Orange and white dotted circles surround cells and nuclei, respectively. Time of mitosis is represented in minutes:seconds. Time 00:00 corresponds to the first NEBD. (C) Graph bars showing the percentage of cells in Ctrl (n = 149 cells from two independent experiments) and PLK4OE (n = 161 cells from two independent experiments) OVCAR-8 cells. Statistical significance by a multiple t test. Error bars represent the mean ± SD, p the P value; ns, not significant.

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