Centrosome clustering takes place in polyploid mitosis, but it is not sufficient to promote bipolar spindle assembly. (A–D) Stills of time-lapse videos of mitotic NBs expressing histone-RFP (red) and tubulin-GFP (A and B) or Ncd-GFP (C and D; green). Orange and white dotted circles surround cells and nuclei, respectively. Time of mitosis is represented in minutes:seconds, and time 00:00 corresponds to NEBD. Schematic representations of mitosis are shown above the stills. Ctrl, ncd^mut(A), polyploid, ncd^mut(B), Ctrl, NcdOE (C), and polyploid, NcdOE (D)NBs. (E) Graph bars showing the percentage of cells undergoing bipolar and multipolar mitosis in Ctrl, ncd^mut (n = 23 NBs from 3 BL), polyploid, ncd^mut (n = 34 NBs from 20 BL), Ctrl, NcdOE (n = 25 from 2 BL), and polyploid, NcdOE (n = 35 from 6 BL) NBs. (F) Dot plot showing the time spent in mitosis for Ctrl, ncd^mut (n = 24 NBs from 3 BL), polyploid, ncd^mut (n = 15 NBs from 12 BL), Ctrl, NcdOE (n = 25 NBs from 2 BL), and polyploid, NcdOE (n = 16 NBs from 5 BL) NBs. (G) XY plot and corresponding linear regression between the number of nuclei generated at anaphase and cell area for polyploid, ncd^mut (n = 34 NBs from 20 BL) and polyploid, NcdOE (n = 35 NBs from 6 BL) NBs. Statistical significance of the correlation was determined by a Spearman r test. r corresponds to the correlation coefficient. (H) Dot plot showing the NI for Ctrl, ncd^mut (n = 24 NBs from 3 BL), polyploid, ncd^mut (n = 14 NBs from 11 BL), Ctrl, NcdOE (n = 25 NBs from 2 BL), and polyploid NcdOE (n = 15 NBs from 4 BL) NBs. (F and H) Statistical significance was determined using a Mann-Whitney test. Error bars represent the mean ± SD (F) and the median ± interquartile range (H). p corresponds to the P value.