Small polyploid NBs without centrosomes divide bipolarly. (A and B) Stills of time-lapse videos of mitotic small (A) and large (B) polyploid, sas4^mut NBs expressing tubulin-GFP (green and gray in the bottom insets) and histone-RFP (red). Orange and white dotted circles surround cells and nuclei, respectively. Time of mitosis is indicated in minutes:seconds. Time 00:00 corresponds to NEBD. Schematic representations of mitosis above the stills. (C) Graph bars showing the percentage of cells in each category in Ctrl, sas4^mut (n = 23 NBs from 5 BL), and polyploid, sas4^mut (n = 56 NBs from 22 BL) NBs. (D) Dot plot showing the time spent in mitosis for Ctrl, sas4^mut (n = 23 NBs from 5 BL), and polyploid, sas4^mut (n = 28 NBs from 14 BL) NBs. (E) XY plot of nuclei at anaphase and cell area (n = 56 NBs from 22 BL). Statistical significance of the correlation by a Spearman r test, r corresponds to the correlation coefficient. (F) Dot plot of NI in Ctrl, sas4^mut (n = 23 NBs from 5 BL), and polyploid, sas4^mut (n = 28 NBs from 14 BL) NBs. (D and F) Statistical significance by a Mann-Whitney test. Error bars represent the mean ± SD (D) and the median ± interquartile range (F), p the P value. ns, not significant.