Polyploid NBs undergo multipolar mitosis. (A and B) Images of whole mount diploid (A) and polyploid (B) brain lobes (BL) and NBs (insets) labeled with antibodies against α-tubulin (red) and Cnn (green). DNA in blue. (C) Dot plot of centrosome number (Nb) per Ctrl (n = 30 NBs from 4 BL) and polyploid (n = 38 NBs from 10 BL) NBs. Statistical significance was determined using a t test. (D–F) Stills of time-lapse videos of mitotic NBs expressing tubulin-GFP (green and gray in the bottom insets) and histone-RFP (red). Orange and white dotted circles surround cells and nuclei, respectively. Time of mitosis is indicated in minutes:seconds. Time 00:00 corresponds to NEBD. Schematic representations above the stills. (D–F) Ctrl diploid (D), small (E), and large (F) polyploid NBs. (G) Percentage of cells in each category in Ctrl (n = 34 NBs from 2 BL) and polyploid NBs (n = 107 NBs from 37 BL). Statistical significance by a multiple t test. Error bars represent the mean ± SD and p the P value.