Supporting information related to Fig. 7 and Fig. 8 . (A) The percentage of NE rupture after transfection with the vector, a dominant-negative KASH2 or an inactive KASH2ext, as scored relative to E4orf4-mRFP-expressing HeLa cells (means ± SEM; n ≥ 3). (B) The percentage of resorbing blebs in cells treated as in A, as scored relative to the total number of recorded blebs in E4orf4-mRFP-expressing HeLa cells, from an 8-h live-cell imaging performed 9 h after transfection (means ± SEM; n ≥ 4). (C) The percentage perinuclear actin remodeling in HeLa cells treated as in A, as scored by actin ring formation relative to E4orf4-mRFP-expressing cells 24 h after transfection (means ± SEM, n = 3). (D) Representative deconvolved single plane confocal images of HeLa cells transfected with GFP-Par3 alone or E4orf4-mRFP; actin was stained with phalloidin. Red dotted lines outline nuclear shape (N), and enlarged views of the boxed regions show Par3-GFP distribution (in green) relative to F-actin organization in the perinuclear region (in red). Bar: 20 µm. (E) Representative epifluorescence images of cells treated with siPar3-3 showing Par3 depletion efficiency in single cells. A heatmap was applied to show the reduction of Par3 signal. Bar: 20 µm. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001; ns, not significant.