Figure S4.
Par3 depletion, as SUN1/2 depletion, impairs E4orf4 tumoricidal phenotypes. (A) Representative IB of Par3 depletion in E4orf4-expressing HeLa cells (siPar3-3 and 3-9), as estimated by loading increasing amounts of control extracts (siCTL, 1, 1/2, and 1/4); GAPDH: loading control. (B and C) Maximum intensity projections of confocal image stacks of control E4orf4-mCherry-expressing HeLa or U2OS cells (siCTL and shCTL), as compared with cells treated with Par3-specific siRNAs (B), or transduced with lentivirus carrying Par3-specific shRNAs (B) or a mixture of SUN1/2-specific shRNAs (C), showing representative phenotypes. Please note that Par3 depletion, as SUN1/2 depletion, inhibited cell blebbing and nuclear condensation/dysmorphia; F-actin staining, green; lamin A/C staining, magenta. Bars: 20 µm; n ≥ 3.

Par3 depletion, as SUN1/2 depletion, impairs E4orf4 tumoricidal phenotypes. (A) Representative IB of Par3 depletion in E4orf4-expressing HeLa cells (siPar3-3 and 3-9), as estimated by loading increasing amounts of control extracts (siCTL, 1, 1/2, and 1/4); GAPDH: loading control. (B and C) Maximum intensity projections of confocal image stacks of control E4orf4-mCherry-expressing HeLa or U2OS cells (siCTL and shCTL), as compared with cells treated with Par3-specific siRNAs (B), or transduced with lentivirus carrying Par3-specific shRNAs (B) or a mixture of SUN1/2-specific shRNAs (C), showing representative phenotypes. Please note that Par3 depletion, as SUN1/2 depletion, inhibited cell blebbing and nuclear condensation/dysmorphia; F-actin staining, green; lamin A/C staining, magenta. Bars: 20 µm; n ≥ 3.

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