Supporting information related to Fig. 4 . (A) Representative merged plane views from confocal image stacks of the PLA signal in controls, as detected when no primary antibodies are used (top row), or when cells are transfected with the empty vector instead of HA-E4orf4 (second row), as compared with the PLA reaction using anti-HA and anti-Par3 antibodies in cells transfected with HA-E4orf4 (third row); from n = 3. Bar: 20 µm. (B) Single plane confocal images of HeLa cells transfected with the empty vector (EV) or Flag-E4orf4-mRFP showing immunostaining of Ser-phosphorylated PKC substrates (in green). Enlarged views of the boxed regions emphasize positive clusters of PKC substrates designated by the arrowheads that are localized next to nuclear blebs. Bar: 20 µm; N.A., not applicable (C) Maximum intensity projections of confocal image stacks of HeLa and U2OS cells showing the loss of tumoricidal phenotypes (cell blebbing and nuclear condensation-deformation) in cells expressing E4orf4 (GGG) as compared with cells expressing E4orf4 (WT); actin staining (green) and lamin A/C staining (magenta) are shown (representative of n ≥ 3); bars, 20 μm.