Lyn-FG and FGH-Ras were recruited at Ab-CTXB-GM1 clusters but not at CTXB-5-GM1. The distributions (histograms) for the colocalization durations are shown. See the Fig. 6 legend for details and keys. See Table S2 for statistical parameters. (A) Lyn-FG was recruited at Ab-CTXB-GM1 clusters but not at CTXB-5-GM1 (a, b). (B) FGH-Ras was recruited at Ab-CTXB-GM1 clusters but not at CTXB-5-GM1 (a, b), and its recruitment at Ab-CTXB-GM1 clusters depended on the PM cholesterol (c). (C) FGH-Ras was not recruited to DNP-DOPE clusters. (D) Summary of the bound lifetimes (τ₂) of Lyn-FG and FGH-Ras at Ab-CTXB-GM1 clusters. ND, τ₂ component not detected.