Requirements for assembly and maintenance of PCM foci in acentriolar cells. (A) Analysis of CEP192 foci formation after mitotic entry in control, nocodazole-treated, or PLK1-inhibited acentriolar cells generated by centrinone treatment. (B) CEP192 foci presence on monopolar spindles generated by PLK1 versus Eg5 inhibition. (C and D) Effect of depolymerizing microtubules (C) or inhibiting PLK1 (D) on maintenance of CEP192 foci formed after mitotic entry in acentriolar cells. Graph on right plots relative intensity of foci over time after inhibitor addition, normalized to the mean value at time 0. Error bars are 95% CI. Scale bars, 10 µm.