Figure S1.
PCNT and CDK5RAP2 knockout cell line generation scheme, genotyping, and analysis of centrosomal CEP192 intensity. (A and B) Schematics of the encoded protein, the genomic locus, and gRNAs employed to target the locus. The antigens for the antibodies used are depicted above the protein cartoon. Genotyping results for the knockout clones employed in the analysis are shown on the right. For CDK5RAP2, a 5-bp insertion in exon 1 and a 3-bp deletion in exon 3 were found in the clones. For PCNT, a single-bp insertion was observed in exon 1. Immunofluorescence and immunoblotting data are shown in Fig. 1 A. (C) Quantification of CEP192 centrosomal intensity in the parental Control (RPE1 USP28Δ) and knockout cell lines. Centrosomal intensity was measured in fixed mitotic cells; for example images, see Fig. 1 B. ****, P < 0.0001 from unpaired t tests. Error bars are 95% CI.

PCNT and CDK5RAP2 knockout cell line generation scheme, genotyping, and analysis of centrosomal CEP192 intensity. (A and B) Schematics of the encoded protein, the genomic locus, and gRNAs employed to target the locus. The antigens for the antibodies used are depicted above the protein cartoon. Genotyping results for the knockout clones employed in the analysis are shown on the right. For CDK5RAP2, a 5-bp insertion in exon 1 and a 3-bp deletion in exon 3 were found in the clones. For PCNT, a single-bp insertion was observed in exon 1. Immunofluorescence and immunoblotting data are shown in Fig. 1 A. (C) Quantification of CEP192 centrosomal intensity in the parental Control (RPE1 USP28Δ) and knockout cell lines. Centrosomal intensity was measured in fixed mitotic cells; for example images, see Fig. 1 B. ****, P < 0.0001 from unpaired t tests. Error bars are 95% CI.

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