Separase localizes normally after KNL-1,3 depletion. Meiotic embryos were fixed and stained with anti-separase (SEP-1), anti-GFP, and DAPI. (A–D) Single bivalents in metaphase I embryos of the indicated genotype. Values for SEP-1 are the mean pixel value of the brightest region around a bivalent ± SEM. Results of Student's t test: control RNAi versus GFP(RNAi), P = 0.037; and AID no auxin control versus AID + auxin, P = 0.71. (E and F) Early anaphase spindles with separase localized in between separating chromosomes. Bars = 2.6 µm.