Figure 2.
Microtubule-free channels are filled with kinetochores.(A and B) Single–focal plane time-lapse sequences of embryos expressing KNL-1::GFP, GFP::KNL-3, and mKate::tubulin. Arrows indicate points of KNL-1,3 extending poleward. (C) Fluorescence intensity profile down the length of a shortened spindle just before anaphase onset. Gray line indicates the half-maximal intensity of mKate::tubulin. The inside edge of the microtubule-free channel was defined as the position of the inner red half-maximal intensity, and the outside edge of KNL was defined as the position of the outer green half-maximal intensity. (D) The length of KNL signal divided by the length of its corresponding microtubule-free channel. Values and error bars indicate mean ± SEM. Bars = 4 µm. ROI, region of interest; TUB, tubulin.

Microtubule-free channels are filled with kinetochores. (A and B) Single–focal plane time-lapse sequences of embryos expressing KNL-1::GFP, GFP::KNL-3, and mKate::tubulin. Arrows indicate points of KNL-1,3 extending poleward. (C) Fluorescence intensity profile down the length of a shortened spindle just before anaphase onset. Gray line indicates the half-maximal intensity of mKate::tubulin. The inside edge of the microtubule-free channel was defined as the position of the inner red half-maximal intensity, and the outside edge of KNL was defined as the position of the outer green half-maximal intensity. (D) The length of KNL signal divided by the length of its corresponding microtubule-free channel. Values and error bars indicate mean ± SEM. Bars = 4 µm. ROI, region of interest; TUB, tubulin.

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