Figure 2.
Cik1 depletion impairs microtubule alignment and kinetochore clustering in metaphase spindles.(A) Cik1-AID cells expressing the microtubule plus-end marker Bik1-3xGFP and the SPB marker Spc42-RFP were imaged in the presence and absence of auxin. Scale bar is 3 µm. Micrograph inserts show 2× magnification of marked spindle. Scale bar is 1 µm. Metaphase spindle morphology in 40 cells was categorized into aligned and misaligned spindles. (B) Analysis of kinetochore position in Cik1-depleted cells. Representative fluorescence images of cells expressing the kinetochore marker Ndc80-GFP and the SPB marker Spc42-RedStar2 are shown. Scale bars are 3 µm. Inserts show 2× magnification of a kinetochore cluster together with the SPBs marked by Spc42-RedStar2. Scale bars in the insets are 3 µm. Kinetochore organization in 40 metaphase spindles was categorized into on-axis and off-axis Ndc80 signals relative to the SPBs.

Cik1 depletion impairs microtubule alignment and kinetochore clustering in metaphase spindles. (A) Cik1-AID cells expressing the microtubule plus-end marker Bik1-3xGFP and the SPB marker Spc42-RFP were imaged in the presence and absence of auxin. Scale bar is 3 µm. Micrograph inserts show 2× magnification of marked spindle. Scale bar is 1 µm. Metaphase spindle morphology in 40 cells was categorized into aligned and misaligned spindles. (B) Analysis of kinetochore position in Cik1-depleted cells. Representative fluorescence images of cells expressing the kinetochore marker Ndc80-GFP and the SPB marker Spc42-RedStar2 are shown. Scale bars are 3 µm. Inserts show 2× magnification of a kinetochore cluster together with the SPBs marked by Spc42-RedStar2. Scale bars in the insets are 3 µm. Kinetochore organization in 40 metaphase spindles was categorized into on-axis and off-axis Ndc80 signals relative to the SPBs.

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