NuMA-N and -C responses in Opto-NuMA in vivo experiments, and purified NuMA truncation proteins used for in vitro EMSAs. (A) Pearson’s correlation coefficient between NuMA-N and/or NuMA-C images of nuclear regions in Opto-NuMA experiments at the indicated times (related to Fig. 4 B). Blue represents illumination with blue light. Lines 1 and 2 show that NuMA-C localization during illumination correlates with NuMA-N localization both before and after illumination, consistent with NuMA-N being recruited to the same NuMA-C localization before and after illumination; line 3 shows that NuMA-C localization does not correlate with NuMA-N localization during illumination, consistent with NuMA-N unbinding from NuMA-C during illumination; line 4 shows that NuMA-N localizations before and during illumination do not correlate; line 5 shows that NuMA-N localizations before and after illumination correlate, consistent with NuMA-N being recruited to the same NuMA-C localization before and after illumination; line 6 shows that NuMA-C localizations at the start and end of illumination correlate. Together these results suggest that NuMA-C drives NuMA-N localization, and that NuMA-C has a higher affinity for chromatin than NuMA-N. The representative cell shown in Fig. 4 B was analyzed. (B) Coomassie blue–stained gels with SNAP-tagged NuMA-N, NuMA-C, NuMA-Bonsai, and NuMA-NC proteins purified from insect Sf9 cells. These purified NuMA truncation proteins were used in EMSAs (Fig. 4 D).