NuMA is only detectable on chromosomes after initial Lap2β recruitment. Representative immunofluorescence images of uninduced RPE1 NuMA KO cells showing localization of the nuclear envelope protein Lap2β (green), NuMA (red), and DNA (Hoechst, blue) over the cell cycle. Lap2β is detectable around the chromosome mass (green arrows) before NuMA appears on chromosomes (red arrows). Scale bar, 5 µm.