Figure 3.
MDC formation requires ERMES and Gem1.(A) Widefield images of WT and the indicated mutant yeast expressing Tom70-GFP and Tim50-mCherry treated with DMSO or rapamycin. Arrows mark MDCs. Images show maximum intensity projections. Scale bars = 2 µm. (B) Quantification of A. Error bars show mean ± SEM of three replicates, n = 100 cells per replicate. (C) Schematic of Gem1 and mutants used in D. (D) Quantification of rapamycin-induced MDC formation in WT and gem1Δ cells expressing EV or overexpressing GEM1 or the indicated GEM1 mutant. Error bars show mean ± SEM of three replicates, n = 100 cells per replicate. (E) Western blot analysis of Gem1 in WT and gem1Δ cells expressing EV or overexpressing GEM1 or the indicated GEM1 mutant. Pgk1 was used as a loading control. Note that the Gem1 antibody did not detect endogenous Gem1 (WT + EV). See also Fig. S2. Rap, rapamycin; TM, transmembrane.

MDC formation requires ERMES and Gem1. (A) Widefield images of WT and the indicated mutant yeast expressing Tom70-GFP and Tim50-mCherry treated with DMSO or rapamycin. Arrows mark MDCs. Images show maximum intensity projections. Scale bars = 2 µm. (B) Quantification of A. Error bars show mean ± SEM of three replicates, n = 100 cells per replicate. (C) Schematic of Gem1 and mutants used in D. (D) Quantification of rapamycin-induced MDC formation in WT and gem1Δ cells expressing EV or overexpressing GEM1 or the indicated GEM1 mutant. Error bars show mean ± SEM of three replicates, n = 100 cells per replicate. (E) Western blot analysis of Gem1 in WT and gem1Δ cells expressing EV or overexpressing GEM1 or the indicated GEM1 mutant. Pgk1 was used as a loading control. Note that the Gem1 antibody did not detect endogenous Gem1 (WT + EV). See also Fig. S2. Rap, rapamycin; TM, transmembrane.

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