NVJs impact NE morphology in nup116ΔGLFG strains. (A) TEM images of cells grown at 30°C. N, nuclei; V, heavily stained vacuoles. Arrowhead points to NE herniation. Scale bars, 500 nm. (B) Quantification of total LD number (left) and LDs at the NE (right) per cell in the listed strains grown at 34°C. Box and whiskers represent 2.5th–97.5th percentile. *, P ≤ 0.01 compared with wild type using Dunn’s post hoc test; **, P ≤ 0.02 when also compared with nup116ΔGLFG mdm1Δ nvj1Δ.