Figure S3.
LDs and Mdm1 are enriched at NE–vacuole interfaces in nup116ΔGLFG cells.(A) FM4-64–stained nup85-GFP or nup85-GFP′ strains grown at 30°C; images were used to quantify data in Fig. 2 F. Scale bar, 5 µm. (B) FM4-64– and Hoechst-stained mdm1-mNeonGreen strains grown at 30°C. Arrowheads point to cells where Mdm1-mNeonGreen localizes around multiple regions of NE–vacuole contacts. Scale bar, 5 µm. (C) Images of FM4-64– and BODIPY-stained wild-type and nup116ΔGLFG cells grown at 34°C. Scale bar, 5 µm. These cells, as well as other independent experiments, were used to quantify data in Fig. 3 F. (D) Number of Pdr16-GFP foci per cell for the listed strains grown at 30°C (n = 120 cells from three independent trials). Box and whiskers represent 2.5th–97.5th percentile; *, P ≤ 0.001 compared with wild type using two-tailed Mann–Whitney U test.

LDs and Mdm1 are enriched at NE–vacuole interfaces in nup116ΔGLFG cells. (A) FM4-64–stained nup85-GFP or nup85-GFP′ strains grown at 30°C; images were used to quantify data in Fig. 2 F. Scale bar, 5 µm. (B) FM4-64– and Hoechst-stained mdm1-mNeonGreen strains grown at 30°C. Arrowheads point to cells where Mdm1-mNeonGreen localizes around multiple regions of NE–vacuole contacts. Scale bar, 5 µm. (C) Images of FM4-64– and BODIPY-stained wild-type and nup116ΔGLFG cells grown at 34°C. Scale bar, 5 µm. These cells, as well as other independent experiments, were used to quantify data in Fig. 3 F. (D) Number of Pdr16-GFP foci per cell for the listed strains grown at 30°C (n = 120 cells from three independent trials). Box and whiskers represent 2.5th–97.5th percentile; *, P ≤ 0.001 compared with wild type using two-tailed Mann–Whitney U test.

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