Figure S8.
Supplemental motility data.(A) Tracking data show cell movements from the experiments in Fig. 4 (the same SMIFH2 and DMSO graphs are shown in Fig. 4 D and reproduced here for comparison purposes). Cell tracks show movement 5 min before (Pre-treat., gray traces) and 5 min after (colored traces) treatment. (B) In addition to the inhibitors shown in Fig. 4, each experimental replicate also included cells treated with jasplakinolide (Jasp), phalloidin, and cytochalasin D (CytoD). Each small gray symbol represents the change in speed of a single cell after treatment, while larger symbols represent the averages from each experimental replicate, coordinated by shape (circles, squares, and triangles). (C) Cells were imaged as in Fig. 4; after 5 min of imaging in buffer, cells were treated with DMSO, SMIFH2, CK-666, or a cocktail of SMIFH2 and CK-666 (SM+CK) and imaged for an additional 5 min. 20 randomly selected cells from the center of the field of view at the time of treatment were tracked to calculate average speeds before and after treatment. Each point represents the speed of a cell before treatment (plotted on the x axis) and after treatment (plotted on the y axis). Three experimental replicates are indicated using different shapes (squares, triangles, and circles). The dashed line has a slope of 1, indicating where cells would fall if the speed was unchanged. (D) The data collected from experiments in C were used to calculate the change in cell speed after treatment. Each smaller gray symbol represents a single cell, while larger symbols represent the averages from each experimental replicate, with shapes representing independent replicates. (E) Directional persistence was calculated for each post-treatment cell tracked in C by dividing the maximum displacement from the start of the track by the total path length. Each small gray symbol represents the persistence of a single cell, while larger symbols represent experimental averages, coordinated by shape. For B, D, and E, black lines indicate the mean ± SD calculated from the three experimental replicates, and statistical significance was determined using an ordinary ANOVA followed by Tukey’s multiple comparison test.Pre-treat., before treatment.

Supplemental motility data. (A) Tracking data show cell movements from the experiments in Fig. 4 (the same SMIFH2 and DMSO graphs are shown in Fig. 4 D and reproduced here for comparison purposes). Cell tracks show movement 5 min before (Pre-treat., gray traces) and 5 min after (colored traces) treatment. (B) In addition to the inhibitors shown in Fig. 4, each experimental replicate also included cells treated with jasplakinolide (Jasp), phalloidin, and cytochalasin D (CytoD). Each small gray symbol represents the change in speed of a single cell after treatment, while larger symbols represent the averages from each experimental replicate, coordinated by shape (circles, squares, and triangles). (C) Cells were imaged as in Fig. 4; after 5 min of imaging in buffer, cells were treated with DMSO, SMIFH2, CK-666, or a cocktail of SMIFH2 and CK-666 (SM+CK) and imaged for an additional 5 min. 20 randomly selected cells from the center of the field of view at the time of treatment were tracked to calculate average speeds before and after treatment. Each point represents the speed of a cell before treatment (plotted on the x axis) and after treatment (plotted on the y axis). Three experimental replicates are indicated using different shapes (squares, triangles, and circles). The dashed line has a slope of 1, indicating where cells would fall if the speed was unchanged. (D) The data collected from experiments in C were used to calculate the change in cell speed after treatment. Each smaller gray symbol represents a single cell, while larger symbols represent the averages from each experimental replicate, with shapes representing independent replicates. (E) Directional persistence was calculated for each post-treatment cell tracked in C by dividing the maximum displacement from the start of the track by the total path length. Each small gray symbol represents the persistence of a single cell, while larger symbols represent experimental averages, coordinated by shape. For B, D, and E, black lines indicate the mean ± SD calculated from the three experimental replicates, and statistical significance was determined using an ordinary ANOVA followed by Tukey’s multiple comparison test.Pre-treat., before treatment.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal