Naegleria species encode an extensive repertoire of actin cytoskeletal regulators. (A) N. gruberi (N.g.) and N. fowleri (N.f.) genomes each encode a similar cellular complement of actin cytoskeletal proteins. The first column shows the name of the protein family or complex, the second includes the name of the mammalian homolog (with the exception of Class II formins, which are often found in plants, and Diaphanous Like Formins [DLF], which encompass all diaphanous-related formins), and the final columns indicate the number of genes found encoding these proteins in N. gruberi and N. fowleri, respectively. See Table S2 for accession numbers and sequences. We note that N. gruberi also possesses five WH2-domain–containing proteins not listed above, and no homolog of WASP Interacting Protein was identified in either Naegleria genome. (B) The graph from Fig. 1 D is shown (with all actins and Joint Genome Institute accession numbers listed) for comparisons of expression data with the proteins listed in A. Each bar represents the average relative change in transcript abundance between amoebae and flagellates from three experiments ± SD.