Actin and microtubule cytoskeletal gene expression differs between Naegleria amoebae and flagellates. (A) The relative transcript abundance for actin cytoskeletal genes (green), microtubule cytoskeletal genes (magenta), and GAPDH (gray) was calculated using expression data collected from amoeba (0 min into differentiation) or flagellate (80 min into differentiation) populations (original data from Fritz-Laylin and Cande, 2010). The relative transcript abundance in flagellates was subtracted from the level in amoebae to generate the graphs shown in Fig. 1, C and D. The microtubule cytoskeletal genes expressed in amoebae include the mitotic tubulins as well as putative spindle components. (B and C) The actin cytoskeletal transcript levels shown in A were organized such that the relative abundances in amoebae (jade green) and flagellates (salmon) were side by side for each of the indicated genes (numbers are in reference to the Joint Genome Institute accession numbers). Actins are shown in the top panel, nucleators and nucleation promoting factors are grouped in the middle panel, and other actin-binding proteins and motors (myosin heavy chains) are shown in the bottom panel. B is set to a linear scale, while C is on a log scale. For all graphs, each bar represents the average relative transcript abundance from three independent experiments ± SD.