Analysis of Crumbs and Par3 distribution. (A–A″′) Cross-section view showing that Crb-GFP (green) localizes just apical to AJs (E-cad, red) in NE cells (white arrows). Crb-GFP is down-regulated in medial-most TZ cells (yellow arrow) and was not detected in NSCs (Dpn, blue). (B and B′) epi-NSCs (Neur-GFP, green) retain Par3 (red) at junctions (see arrows), albeit at a slightly lower level consistent with junction remodeling in epi-NSCs. Scale bars = 10 µm.