Comparison of Cry2WT-mCherry-Tau distribution before and after light activation for various Tau constructs. (A–J) Cry2WT-mCherry fused various Tau constructs was overexpressed in SH SY5Y cells. Signal distribution was studied before and after blue light. Representative maximum intensity Z-projection images before and after blue light of each construct are shown, CWT 1–254 before (A) and after (B), CWT 1–281 before (C) and after (D), CWT 1–311 before (E) and after (F), CWT 1–343 before (G) and after (H), and CWT 1–375 before (I) and after (J). Scale bar, 10 µm. (K–M) Quantifications of Cry2WT-mCherry-Tau distribution before activation (K), after activation (L), and change by activation (M) are shown in boxplot. Constructs from left to right along the x axis are CWT 244–375, CWT 151–375, CWT 1–441, CWT 1–254, CWT 1–281, CWT 1–311, CWT 1–343, and CWT 1–375. Distribution of Cry2WT-mCherry-Tau is evaluated by pixel CV over the cell shown along the y axis. n = 19, 16, 23, 8, 16, 15, 9, and 20 for constructs from left to right along the x axis. Mean for each group is indicated by a purple square, also listed in Table S1. Base-mean for all the constructs as a whole is indicated as a horizontal dotted line. Statistical significance by pairwise t test of each independent group is indicated at the top of each plot, from top to bottom, against all the constructs as a whole, against CWT 1–441, and against CWT 151–375, correspondingly. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.