Cry2WT-mCherry-Tau fragments associate with MT. (A) Association of PRD condensates with MTs. EGFP-α-tubulin (green) and CWT 151–254 (red) were coexpressed in neuroblastoma SH SY5Y cells. Time-lapse images show CWT 151–254 forms LLPS upon blue-light activation starting from the second time frame. Montage of two fluorescent channels in the third time frame demonstrates CWT 151–254 foci are attached to EGFP-α-tubulin on MT, like beads on a thread (blue arrow). (B–G) Cry2WT-mCherry fused to MTBD without and with PRD were overexpressed in SH SY5Y cells, and signal distribution was studied before and after blue light. Representative maximum intensity Z-projection images before and after blue light of each construct are shown, CWT 1–441 before (B) and after (C), CWT 244–375 before (D) and after (E), CWT 151–375 before (F) and after (G). Insets (scale bar, 5 µm) within the white rectangle show more details of the diffuse pool and the MT-binding population in each construct. Scale bar unless specifically mentioned, 10 µm.