Proximity to sites of exocytosis and cell polarity signaling influences the rate of endocytic site maturation. (A) Montage from a maximum-intensity–projected epifluorescence video of a cell endogenously expressing Sla1-mCherry (magenta) and Ede1-GFP (green). Times are hours:minutes. See Video 2. (B) Maximum-intensity projections of z-stacks (left) of polarized and depolarized cells paired with circumferential kymographs around the mother cortex from medial focal plane videos of the same cells (right) endogenously expressing Sla1-mCherry (magenta) and Ede1-GFP (green). See Video 3. (C) Quantification of the ratio of the number of Sla1-mCherry sites to Ede1-GFP sites in mother cells from maximum-intensity projections of z-stacks of 60 polarized and 60 depolarized cells. A two-tailed P value from a Mann–Whitney U test with the null hypothesis that the two ratios are identical (U = 1) is displayed. The median and interquartile ranges are denoted with error bars. (D) Percentage of 596 Ede1 patches from 59 polarized mother cells and 1,034 Ede1 patches from 56 depolarized mother cells that persist throughout the duration of a 4-min video (persistent), that are present at the start or end of the video (partial), or that assemble and disassemble within the interval of the video (turnover). Numbers indicate the percentage of patches observed in each category. A two-tailed P value from a chi-square test with the null hypothesis that the proportion distributions are identical (chi-square = 1,349, 2 degrees of freedom) is displayed.