Figure 6.

CME protein lifetimes are correlated among earlier-arriving proteins, but early protein lifetimes are not correlated with late protein lifetimes. (A–E) Scatter plots of Ede1-RFP lifetime (Life.) vs. query protein lifetime for GFP-tagged Syp1 (A), Hrr25 (3X-GFP tagged; B), Pal1 (C), and Apl1 (D), with an accompanying summary of linear fits (E) to the plots as in Fig. 3 D. (F–K) Scatter plots of Sla1-mCherry lifetime vs. query protein lifetime for GFP-tagged Ede1 (F), Syp1 (G), Hrr25 (3X-GFP tagged; H), Pal1 (I), and Apl1 (J), with an accompanying summary of linear fits (K) to the plots as in Fig. 3 D. Color in scatter plots indicates the normalized data density in the neighborhood around each point. Red lines are linear fits to the data with the indicated R2 value. Labels for bar plots in E and K are purple in agreement with the color scheme in previous figures, to indicate that all proteins queried are early proteins.

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