Septin 7 primarily accumulates within the cell cortex away from the CS. (A) NKL cells were allowed to form conjugates with CMAC-labeled 721.221 cells (blue) and stained for F-actin (red) or septin 7 (green) and imaged using confocal microscopy. Scale bar, 5 µm. (B) Line intensities profiles were created from the aqua line at the CS and magenta line along the NK cell cortex in images from A using Fiji. (C) Primary human NK cells were nucleofected with either control or septin 7–1 siRNA. 72 h after nucleofection, cells were placed on to poly-L-lysine–coated coverslips, fixed, stained with anti–septin 7, and imaged by confocal microscopy. Dotted lines in the bottom panel denote the outline of the NK cell. Scale bar, 10 µm.