Septins are DOCK8 interacting proteins. (A) Primary NK cells were lysed and immunoprecipitated with anti-DOCK8 antibody or rabbit IgG and immunoblotted for the indicated septin proteins and DOCK8. (B and C) Primary NK cells were stimulated over the indicated time course by ligation of CD16, the low-affinity FcγRIIIa receptor (B), or NKG2D/2B4 receptors (C), and the indicated proteins were immunoprecipitated and immunoblotted. Representative examples of three independent experiments are shown. rIgG, rabbit IgG.