Figure 3.
p53 promotes DNA repair in response to acute DNA damage in E1A;HrasG12V;H11Cas9 MEFs.(A) Schematic of the γIR of E1A;HrasG12V MEFs and subsequent analysis of DNA repair over time. (B) Representative images of γH2AX foci in E1A;HrasG12V MEFs at different times after IR. DAPI marks nuclei. Scale bar, 10 μm. (C) Average percentage of γH2AXhigh cells ± SD, n = 3 cell lines/sgRNA with 40–105 cells analyzed per cell line/time point. **, P < 0.005; ***, P < 0.0005, two-way ANOVA, Dunnett’s multiple comparison test. For A–C, all experiments were performed in physiological (5%) oxygen.

p53 promotes DNA repair in response to acute DNA damage in E1A;HrasG12V;H11Cas9 MEFs. (A) Schematic of the γIR of E1A;HrasG12V MEFs and subsequent analysis of DNA repair over time. (B) Representative images of γH2AX foci in E1A;HrasG12V MEFs at different times after IR. DAPI marks nuclei. Scale bar, 10 μm. (C) Average percentage of γH2AXhigh cells ± SD, n = 3 cell lines/sgRNA with 40–105 cells analyzed per cell line/time point. **, P < 0.005; ***, P < 0.0005, two-way ANOVA, Dunnett’s multiple comparison test. For A–C, all experiments were performed in physiological (5%) oxygen.

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