Figure 2.
DNA damage signaling is detected in the keratinocyte transition proliferation/differentiation.(a and b) Double IF for γH2AX (green) and the differentiation markers Inv (a, red) or K16 (b, red). Arrows point at the active front of the colony. Bar graph in the right panel displays the average number of γH2AX foci per nucleus in Inv (a) or K16 (b) negative (white) or positive (red) cells. Data are mean ± SEM of representative experiments. Datasets were compared by an unpaired t test (two-sided). P < 0.0001. (c) Double IF for p-Res (green) and Inv (red). Arrows point at stratifying Inv-positive cells strong for p-Res. Arrowhead basal cells dull for p-Res. (d and e) Double IF for p-Res (red) and γ-tubulin (ytub, green) localizing to interphasic (d) and mitotic (e) centrosomes (arrows). Scale bar, 50 µm. Nuclear DNA in blue by DAPI. See also Fig. S1 and Fig. S2.

DNA damage signaling is detected in the keratinocyte transition proliferation/differentiation. (a and b) Double IF for γH2AX (green) and the differentiation markers Inv (a, red) or K16 (b, red). Arrows point at the active front of the colony. Bar graph in the right panel displays the average number of γH2AX foci per nucleus in Inv (a) or K16 (b) negative (white) or positive (red) cells. Data are mean ± SEM of representative experiments. Datasets were compared by an unpaired t test (two-sided). P < 0.0001. (c) Double IF for p-Res (green) and Inv (red). Arrows point at stratifying Inv-positive cells strong for p-Res. Arrowhead basal cells dull for p-Res. (d and e) Double IF for p-Res (red) and γ-tubulin (ytub, green) localizing to interphasic (d) and mitotic (e) centrosomes (arrows). Scale bar, 50 µm. Nuclear DNA in blue by DAPI. See also Fig. S1 and Fig. S2.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal