Labeling cellular microtubules using A1aY1 fused to TagBFP/TagRFP-T. Z-projection of confocal image stacks of U2OS cells stably expressing A1aY1 binder tagged with TagBFP (A) or TagRFP-T (B) at the N terminus, in cyan and magenta, respectively. The cells were additionally stained with SiR-tubulin (in green). The zoom panel shows a closer view of microtubules labeled with A1aY1 TagBFP and TagRFP-T as indicated. The colocalization colormap between A1aY1 binder versus SiR-tubulin is based on a color scale in which negative normalized mean deviation product values are represented by cold colors (segregation); values above 0 are represented by hot colors (colocalization). Scale bars = 10 µm and 5 µm for the whole cell and zoomed panel, respectively. MT, microtubule. Icorr respresents index of correlation measured from the colormap Fiji plugin.