Figure 2.
Centrosomal shifts do not correlate with THN migration speed increases.(A) Centrin-2 labeling illustrates the unstable centrosomal position (arrows) in phase 2 THNs relative to the nucleus. Elapsed time in minutes. Scale bar, 10 µm. See also Video 2. (B) Determining THN speed in a 30-min window centered around a centrosomal position shift (labeled “shift”) compared with a time window preceding (“before”) or following (“after”) does not indicate a speed increase correlation with centrosomal position using either Cetn2 or Map4MTB as markers. When THNs were depolarized by ChR2 or ACh, speed increased irrespective of centrosomal position. Note that the speed increase in Map4MTB was not statistically significant (Kruskal–Wallis ANOVA P = 0.126 before/shift, P = 0.075 shift/after for uninduced, P = 0.753 before/shift, P = 0.165 shift/after for ChR2-induced Map4MTB-expressing THNs). n = 10 embryos/66 tracks/45 events for control Cetn2-expressing THNs; n = 11 embryos/87 tracks/53 events for ACh-induced Cetn2-expressing THNs; n = 6 embryos/26 tracks/38 events for control Map4MTB-expressing THNs; n = 7 embryos/40 tracks/23 events for ChR2-induced Map4MTB-expressing THNs. (C) Plotting the data linked for a specific event does not suggest a preference of speed increases around the time of a centrosome shift (red) over other speed profiles (blue) using Cetn2 as marker. n = 10 embryos/66 tracks/17 events. (D) Map4MTB as marker can be used to determine the MTOC position (arrows) relative to the nucleus (dots). Scale bar, 10 µm. (E) No correlation over statistic background was detected in linked THN speed profiles for speed increases around the time of a centrosomal shift using Map4MTB as marker. n = 6 embryos/26 tracks/27 events for control Map4MTB-expressing THNs. Boxes in graphs represent 25–75% of all values and whiskers 1.5 times the quartile. Median is shown as a horizontal bar and mean as a square box.

Centrosomal shifts do not correlate with THN migration speed increases. (A) Centrin-2 labeling illustrates the unstable centrosomal position (arrows) in phase 2 THNs relative to the nucleus. Elapsed time in minutes. Scale bar, 10 µm. See also Video 2. (B) Determining THN speed in a 30-min window centered around a centrosomal position shift (labeled “shift”) compared with a time window preceding (“before”) or following (“after”) does not indicate a speed increase correlation with centrosomal position using either Cetn2 or Map4MTB as markers. When THNs were depolarized by ChR2 or ACh, speed increased irrespective of centrosomal position. Note that the speed increase in Map4MTB was not statistically significant (Kruskal–Wallis ANOVA P = 0.126 before/shift, P = 0.075 shift/after for uninduced, P = 0.753 before/shift, P = 0.165 shift/after for ChR2-induced Map4MTB-expressing THNs). n = 10 embryos/66 tracks/45 events for control Cetn2-expressing THNs; n = 11 embryos/87 tracks/53 events for ACh-induced Cetn2-expressing THNs; n = 6 embryos/26 tracks/38 events for control Map4MTB-expressing THNs; n = 7 embryos/40 tracks/23 events for ChR2-induced Map4MTB-expressing THNs. (C) Plotting the data linked for a specific event does not suggest a preference of speed increases around the time of a centrosome shift (red) over other speed profiles (blue) using Cetn2 as marker. n = 10 embryos/66 tracks/17 events. (D) Map4MTB as marker can be used to determine the MTOC position (arrows) relative to the nucleus (dots). Scale bar, 10 µm. (E) No correlation over statistic background was detected in linked THN speed profiles for speed increases around the time of a centrosomal shift using Map4MTB as marker. n = 6 embryos/26 tracks/27 events for control Map4MTB-expressing THNs. Boxes in graphs represent 25–75% of all values and whiskers 1.5 times the quartile. Median is shown as a horizontal bar and mean as a square box.

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