Summary performance of YOLOv2 when trained on multichannel data versus single-channel data. (A) Erythroblast cells stained with DAPI (blue) and for glycophorin A protein (green). (B) Neuroblastoma cells stained with phalloidin (green) and DAPI (blue). Scale bars represent 25 µm in both images. All training material includes vertically flipped data augmentation, and the ground-true for the first channel (DAPI) was accessed in both cases. (C) AP of YOLOv2, comparing performance when trained and evaluated on a single dataset comprising one-channel data (a) and two-channel data (b) or when trained on multiple data comprising two channels (c). Erythroblast DAPI glycophorin A dataset (blue) and neuroblastoma phalloidin DAPI dataset (magenta; n = 3, mean ± SD).