Example fluorescence microscopy data generated for our study with corresponding ground-truth human annotations and object detection predictions. All object detections >0.5 confidence are shown. (A and B) Eukaryotic cell dataset, fluorescently stained with DAPI. (C and D) Neuroblastoma cells fluorescently stained with GFP-phalloidin. (E and F) HEK cells fluorescently expressing GFP-SCP2 protein. Ground-truth boxes (white), YOLOv2 prediction boxes (red), and Faster-RCNN prediction boxes (green). Scale bars, 25 µm.