rhogap19d mutant cells are more motile than wild-type cells and contract laterally. (A) Single frames and a temporal projection of a time-lapse movie of a stage 7 egg chamber containing a large rhogap19d mutant clone (marked by the loss of RFP; magenta) and expressing GFP-aPKC (green). The blurred apical surfaces of the mutant cells in the temporal projection indicate that they are moving between frames. (B) Magnification of the boxed areas in A, showing that rhogap19d mutant cells (bottom panels) are more motile (white arrows) than wild-type cells (top panels). Temporal projections after 1, 2, and 4 min. (C) A single frame from a movie showing a cluster of rhogap19d mutant cells (white arrow; marked by the loss of RFP; magenta) beginning to invade the germline. The cells in the cluster appear to have detached from the basement membrane (cell mask; white). The lower panel shows a magnification of the boxed area. (D)rhogap19d mutant cells have lateral foci of NMYII foci (Zipper-GFP; green) and reduced levels at the apical side compared with wild-type cells (white arrows; DAPI, blue). This phenotype was observed in 154 of 157 mutant cells. (E and F) Gek (green) localizes apically in wild-type follicle cells (E) but extends along the lateral domain of all rhogap19d mutant cells (F; DAPI, blue). Scale bars, 10 µm. n = 11 homozygous mutant egg chambers.