rhogap19d mutant cells expand apically in different epithelia. (A and B) Accessory glands from virgin wild-type (A) and rhogap19d mutant males (A) stained for Lgl (green), F-actin (phalloidin; red), and DNA (DAPI; blue). The apical surfaces of the mutant cells protrude into domes, and the cells are much taller than in wild type (n = 11 males). Scale bars, 10 µm. (C and D) Cellular blastoderm embryos (3 h after fertilization) from wild-type (C) and rhogap19d mutant mothers, stained for Dlg (green) and DNA (DAPI; blue). The cells in the embryos laid by homozygous mutant females bulge apically. The vertical arrow and arrowhead in A and C indicate the smooth apical surface in wild type, and the diagonal arrows mark the domed apical surfaces in rhogap19d mutant cells. n = 8 embryos in each background. Scale bars, 10 µm.