rhogap19D mutant cells are taller than wild-type cells and have an enlarged apical domain. (A–E) Regions of the stage 7 follicle cell epithelium stained with DAPI (blue) containing clones of rhogap19d mutant cells marked by the loss of RFP (magenta). (A) Cell mask staining (green) of the plasma membranes reveals that mutant cells are taller than wild-type cells and have domed apical surfaces. The arrows indicate the apical surfaces of the mutant cells. (B) The adherens junctions marked by endogenously tagged E-cadherin (ECad)-GFP (green) form at the same level in rhogap19d mutant and wild-type cells (phalloidin; white). (C) In rhogap19d mutant cells, GFP-aPKC localizes all around the apical domain above the adherens junctions (marked by Cno staining; white; indicated by the white arrow). (D) Crb-GFP marks an enlarged subapical region in rhogap19d cells. (E)rhogap19d mutant cells have slightly shorter lateral domains than wild-type cells, as shown by Lgl-GFP localization (green; white arrow). (F) A graph showing the mean cell height in wild-type and rhogap19d mutant cells. (G) A graph showing the mean cell width in wild-type and rhogap19d mutant cells. (H) A graph showing the mean apical domain length in wild-type and rhogap19d mutant cells. (I) A graph showing the mean lateral domain length in wild-type and rhogap19d mutant cells. The error bars represent SEM; ****, P < 0.0001; **, P < 0.002. Scale bars, 10 µm.