RhoGAP19D inhibits CDC42 activity laterally. (A) The CDC42 effector, N-WASP (tagged with mNeonGreen), spreads laterally in rhogap19D mutant cells (marked by the loss of RFP; magenta, white arrows). This phenotype was observed in 20 of 27 mutant cells. (B) A horizontal section through a lateral region of the follicular epithelium containing a rhogap19D mutant clone marked by the loss of RFP (red), expressing mNeonGreen- N-WASP. N-WASP localizes to the lateral membrane of the mutant cells. (C) Cartoon showing the UAS-GrabFP-Aint system. (D) mCherry-GrabFP-Aint predominantly localizes to the apical side of the follicular cells when expressed under the control of TJ-Gal4, whereas GFP-RhoGAP19D alone localizes laterally (white arrow). (E) Coexpression of mCherry-GrabFP-Aint and GFP-RhoGAP19D results in the apical recruitment of RhoGAP19D, leading to a loss of epithelial polarity and mislocalization of aPKC (white arrow). Scale bars, 10 µm.
