RhoGAP localizes laterally in multiple epithelia. (A and B) A top view (A) and a cross-section (B) through a salivary gland from an L3 larva, showing mNeonGreen-RhoGAP19D (green) localization to the lateral domain. DAPI (blue), n = 8 larvae. (C and D) A top view (C) and a cross-section (D) through an accessory gland from a mated male. mNeonGreen-RhoGAP19D (green) localizes laterally. DAPI (blue), n = 10 males. (E and F) A top view (E) and a cross-section (F) through a live 3-h-old embryo. mNenonGreen-RhoGAP19D (green) localizes laterally, *Autofluorescence of the vitelline membrane. n = 11 embryos. (G) Lateral mNeonGreen-RhoGAP19D (green) localization in the anterior midgut of an L3 larva; n = 3 guts. (H–J) Wild-type localization of mNeonGreen-RhoGAP19D at the lateral cortex of stage 7 follicle cells in which fasII (H; n = 21), fasIII (I; n = 19), and nrg (J; n = 25) have been knocked down using the UAS-RNAi Flp out system (cells that express RFP coexpress the RNAi constructs). Each experiment was performed three times. (K) A stage 8 egg chamber with a clone of cells mutant for N-cadherin1 and N-cadherin2 (marked by the loss of RFP). mNeonGreen-RhoGAP19D localizes normally in the mutant cells (n = 15). The experiment was performed twice. (L) mNeonGreen-RhoGAP19D localizes to the cortex in aPKCHC mutant clones (marked by the loss of RFP) at stage 8. n = 16; experiment performed twice. (M and N) mNeonGreen-RhoGAP19D is correctly localized in cells treated with RNAi against Scrib (n = 23; M) or Coracle (N; n = 16). Stage 8; experiments performed twice. Scale bars, 10 µm.
