Figure 1.
ER and LD morphology in fitm-2 mutant.(A) Representative 3D-SIM images of wild-type and fitm-2(dt002) animals carrying the qxIs439 (Phyp7::gfp::tram-1) transgene at the young adult stage, showing ER morphology in the hypodermis. Scale bar, 10 µm. (B) ER morphology of early embryos and oocytes, shown as single confocal planes, in wild-type and fitm-2(erm002) animals carrying the ojIs23 (Ppie-1::gfp::sp12) transgene. Scale bar, 10 µm. (C) Topology of FITM-2. The blue dot indicates the predicted enzyme active site His-172 in C. elegans. The red dot indicates the mutation site in fitm-2(erm002). (D) LipidTOX staining of larval stage L4 animals. Images are 3D projections of 7.5-µm confocal z-stacks that covered the posterior body forward from the vent. Scale bar, 10 µm. (E) Electron micrographs of the middle gut of the indicated young adult animals. Yellow arrows indicate LDs. Scale bar, 4 µm.

ER and LD morphology in fitm-2 mutant. (A) Representative 3D-SIM images of wild-type and fitm-2(dt002) animals carrying the qxIs439 (Phyp7::gfp::tram-1) transgene at the young adult stage, showing ER morphology in the hypodermis. Scale bar, 10 µm. (B) ER morphology of early embryos and oocytes, shown as single confocal planes, in wild-type and fitm-2(erm002) animals carrying the ojIs23 (Ppie-1::gfp::sp12) transgene. Scale bar, 10 µm. (C) Topology of FITM-2. The blue dot indicates the predicted enzyme active site His-172 in C. elegans. The red dot indicates the mutation site in fitm-2(erm002). (D) LipidTOX staining of larval stage L4 animals. Images are 3D projections of 7.5-µm confocal z-stacks that covered the posterior body forward from the vent. Scale bar, 10 µm. (E) Electron micrographs of the middle gut of the indicated young adult animals. Yellow arrows indicate LDs. Scale bar, 4 µm.

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