Aneuploidy and karyotypic heterogeneity in cells derived from DLD1^CENP-A xenograft tumors and cultured DLD1^CENP-A cells with CENP-A OE. (A) Probes used for miFISH experiments. Representative image of one cell hybridized with miFISH panels 1 and 2. The top row depicts the first hybridization, while the row underneath shows the second hybridization. The merged nuclei on the left of each row shows the overlay of all channels. (B) Cells derived from DLD1^CENP-A xenograft tumors exhibit aneuploidy and karyotypic heterogeneity. Color displays for miFISH with 10 probes depict increased aneuploidy and karyotypic heterogeneity in cells derived from xenograft-induced tumor from mouse fed with DOX (MDX#07) compared with CON (MDX#10) diet. (C) Constitutive CENP-A OE contribute to aneuploidy and karyotypic heterogeneity in cultured DLD1^CENP-A cells. miFISH analysis as in B of control DLD1^CENP-A cells or DLD1^CENP-A cells treated with 0.1 µg/ml DOX for 4 d. DOX was replenished every 2 d for constitutive overexpression of YFP–CENP-A. The color scheme for each color display is as follows: green, gains; red, losses; and blue, unchanged, as determined by comparing each cell to the average ploidy. Nuclei are plotted vertically by pattern frequency. The gain and loss column refers to the percentage of nuclei where a gain or loss was observed with the 10 different probes.