Higher invasion of cells with CENP-A OE. (A) Experimental regimen for invasion assay; see Materials and methods section for detail. (B) Higher invasion in DLD1 cells with overexpressed CENP-A. Images show the invasion of DLD1^CENP-A cells with or without DOX treatment across the trans-well membrane. Insets correspond to white-boxed areas in main images. Yellow arrows show invaded cells. (C) Quantification shows fold increase of invading DLD1^CENP-A cells treated as in D. Error bars represent the SD from four independent experiments. P values calculated by using unpaired Student’s t test are indicated. (D) Depletion of histone chaperone DAXX suppresses the higher invasion in DLD1 cells with overexpressed CENP-A. Western blots show the protein levels of DAXX and YFP–CENP-A in cell lysates of DLD1^CENP-A cells with or without DOX treatment, followed by treatments with siRNA oligoes as indicated. GAPDH was used as loading control. (E) Quantification shows the number of invading DLD1^CENP-A cells with or without DOX treatment, followed by treatments with siRNA oligos as indicated and calculated as in C. Error bars represent the SD from three independent experiments. P values calculated by using unpaired Student’s t test are indicated. Ctrl, control.