Reduced expression of cell adhesion genes in cells with CENP-A OE. (A) Volcano plot of log2 fold-change (log2FC) versus adjusted P value for gene expression changes in DLD1^CENP-A cells with DOX treatment over DLD1^CENP-A cells without DOX treatment. Area enclosed in yellow dashed line shows the spread of data with P value > 0.1. (B) Heat map of GSEA normalized enrichment scores (NES) across two datasets. Log2FC values of gene expression changes were used as input for GSEA of gene sets from Reactome and KEGG databases. Plot shows NES of terms significantly enriched in the dataset from this study (Shrestha) downregulated at FDR < 0.25 or upregulated at FDR < 0.005 and corresponding NES for same terms from Lacoste et al. (2014) with HeLa cells constitutively OE YFP–CENP-A data set. Line in color key represents density plot of NES scores across the two data sets. (C) GSEA enrichment plots for select terms enriched in Shrestha (current study) and Lacoste et al. (2014) data sets. Genes upregulated upon CENP-A OE, such as mitotic genes, are to the left (shaded red) and genes downregulated, such as adherens junction interactions, are to the right (shaded blue). Green line shows walking enrichment score. Black vertical bars represent hits from gene set. (D) Expression of candidate kinetochore genes are not upregulated in response to CENP-A OE. Normalized read counts generated by EdgeR for select CENP and kinetochore proteins. Error bars represent mean ± SD. log2FC < |0.28| and adjusted P > 0.99 for all genes shown, except CENP-A.