CENP-A OE contributes to reduced kinetochore localization of CENP-T, NUF2, and Mis12 in DLD1^CENP-A cells. (A) Reduced centromeric CENP-T signal intensities in DLD1^CENP-A cells with overexpressed and mislocalized CENP-A. Images of CENP-T localization at centromeres on metaphase plates in DLD1^CENP-A cells with or without DOX treatment. (B) Reduced kinetochore NUF2 signal intensities in DLD1^CENP-A cells with overexpressed and mislocalized CENP-A. Images of NUF2 localization at kinetochores on metaphase plates in DLD1^CENP-A cells with or without DOX treatment. (C) Reduced centromeric localization of Mis12 in DLD1^CENP-A cells with overexpressed and mislocalized CENP-A. Images of localization of Mis12 at kinetochores on metaphase plates in DLD1^CENP-A cells with or without DOX treatment. For all panels in this figure, before immunostaining, cells were treated with 10 µM MG132 for 90 min, followed by fixation with ice-cold methanol for 1 min and immunostaining with antibodies as indicated. Cells were stained with DAPI. Scale bar: 5 µm.