Ano5 but not Ano6 mRNA is increased during myogenesis, and Ano6 expression is not changed in myocytes or myotubes from ANO5-KO mice. (A) mRNA expression of Ano5 in WT or ANO5-KO mouse MPCs differentiated for 18 h (myocytes, left) or 72 h (myotubes, right). *, P < 0.05; **, P < 0.01. (B) mRNA expression of Ano6 in WT or ANO5-KO mouse MPCs differentiated for 18 h (myocytes, left) or 72 h (myotubes, right). WT n = 3, ANO5-KO n = 2. Data are mean ± SEM.